IMMUNOHISTOCHEMICAL WET BOX
An immunohistochemical wet box, also known as a humidity chamber, is a container used during immunohistochemical staining procedures to maintain a humid environment while slides are being incubated. A humidity chamber can be made using a plastic box with a lid and wet paper towels layered at the bottom of the box. To create raised platforms for the slides to sit on for ease of access during immunostaining, pipette tubes can be cut to the width of the box and taped in pairs to the bottom of the box.
APPLICATIONS OF IMMUNOHISTOCHEMICAL WET BOX IN RESEARCH AND DIAGNOSTICS
PRIMARY ANTIBODY INCUBATION
During primary antibody incubation, slides must be placed into a wet box, typically for 1-2 hours at 37°C. The humidity prevents evaporation of the antibody solution and ensures consistent antibody binding across the entire tissue section.
PREVENTING TISSUE DRYING
A critical requirement during IHC procedures is that "the section should not dry at any step of IHC." The wet box maintains the necessary humidity to prevent tissue drying, which can cause artifacts, false positives, or inconsistent staining.
OPTIMIZING BLOCKING STEPS
During the blocking of nonspecific binding sites, tissue sections need to be incubated in blocking buffer for 1-2 hours at room temperature. The humidifying chamber with wet tissue paper provides the ideal environment for this critical step that reduces background staining.
OVERNIGHT INCUBATIONS
For extended incubation periods, such as overnight primary antibody incubation at 4°C, the wet box prevents evaporation and maintains reagent concentration throughout the extended period, which is essential for consistent staining results.
MULTIPLE SAMPLE PROCESSING
The design of the humidity chamber allows for multiple slides to be processed simultaneously under identical conditions. This makes it possible to simultaneously run experimental samples alongside appropriate controls, ensuring experimental validity.
TECHNICAL PARAMETERS |
Product Name |
Immunohistochemical Wet Box |
Keywords |
Disposable Medical Supplies |
Size |
Customized |
Color |
Customized |
Application |
Laboratory |
OEM & ODM Custom |
Accept |
Sample |
Accept |
LOGO Custom |
Accept |
Payment |
T/T, L/C, Western Union |
OPTIMIZED HUMIDITY CONTROL
The wet box maintains precise humidity levels during incubation periods, preventing sample drying and ensuring antibodies remain properly hydrated throughout the binding process. This is crucial when slides need to incubate at 37°C for 1-2 hours or even overnight at 4°C.
ENHANCED STAINING CONSISTENCY
The controlled environment ensures optimal immunohistochemistry results by eliminating variables that can affect antigen-antibody interactions. This leads to more reliable and consistent staining patterns, which is especially critical for diagnostic applications and research requiring quantitative analysis.
MULTI-SAMPLE CAPACITY
Wet Box accommodates multiple slides simultaneously, improving laboratory throughput and workflow efficiency. This is particularly valuable for high-volume testing facilities and research laboratories processing numerous samples.
REDUCED REAGENT CONSUMPTION
The sealed environment of the Wet Box minimizes evaporation, allowing for more efficient use of costly antibodies and detection reagents. This results in significant cost savings while maintaining high-quality staining results during sample preparation.
VERSATILE APPLICATION SUPPORT
The system supports a wide range of IHC methods, making it adaptable to different specimen types and assay sensitivities. This versatility is essential as IHC is widely used in research and clinical laboratories to visualize the distribution and localization of specific cellular components within their proper tissue context.







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